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INDUSTRIAL DIPSLIDES Solar - Cult® Industrial Dipslides Nutrient Agar with TTC ( Tri-phenly tetrazolium chloride) / Rose Bengal Agar Rose Bengal Agar Description Rose-Bengal Chloramphenicol Agar is a selective medium for the enumeration of yeasts and moulds from a wide variety of foodstuffs. The medium has a neutral pH and chloramphenicol is used as a selective agent to suppress the growth of bacteria. Several investigators have noted advantages in the use of media at neutral pH containing antibiotics1,2. Rose-Bengal is taken up by mould and yeast colonies thereby assisting enumeration of small colonies3. Rose-Bengal also controls the size and height of mould colonies, such as Neurospora and Rhizopus spp. Over-growth of slow growing strains by more luxuriant species is thus prevented and plate counting is assisted. Nutrient Agar with TTC ( Triphenyltetrazolium Chloride) Description A general purpose agar medium, containing two peptones, which will support the growth of a wide variety of organisms. It is suitable for the cultivation both of aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions.
A small quantity of the dye 2,3,5- triphenyltetrazolium (TTC) is added. Aerobic bacteria species grow on this medium and they can be detected by their ability to reduce TTC to a red colored formozan dye. Bacterial colonies appear as red dots on an otherwise clear colorless medium, For full details, the original papers should be consulted.
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