CLINICAL DIPSLIDES

Solar - Cult® Clinical Dipslide

CLED AGAR/ MacConkey AGAR

Our MC-200 CLED Agar/ MacConkey Agar Dipslides are coated with growth media on both sides to increase surface contact with the specimen and enable the use of two different media on each slide. Use of different selective growth media permits simultaneous exposure of a urine specimen to a medium selective for gram-positive species on one side, for example, and to a medium selective for gram-negative species on the other.

MacConkey Agar

Description:

A more selective modification of MacConkey medium which is suitable for the detection and enumeration of coliform organisms and also for the detection and isolation of Salmonella and Shigella species occurring in pathological and food specimens. Due to the inclusion of a specially prepared fraction of bile salts in addition to crystal violet, the medium gives improved differentiation between coliforms and non-lactose fermenting organisms whilst Gram-positive cocci are completely inhibited.

CLED Agar

Description:

A dehydrated Cystine-Lactose-Electrolyte Deficient (CLED) medium made to the formula described by Mackey and Sandys1 as a modification for urinary bacteriology of the Electrolyte Deficient Medium developed by Sandys².

This medium is recommended for urinary bacteriology, supporting the growth of all urinary pathogens and giving good colonial differentiations and clear diagnostic characteristics. The presence of important contaminants such as diphtheroids, lactobacilli and micrococci is also clearly elicited, giving an indication of the degree of contamination.

It is electrolyte deficient to prevent the swarming of Proteus species. The medium has been used successfully in the Dip-inoculum Transport Medium technique ( Mackey and Sandys^1,3).

A variant of this technique has been described by Guttman and Naylor⁴ who employed media-coated slides. These techniques overcome false bacteriological results associated with delay in the transport of the specimens of urine to the laboratory and permit a clinically accurate routine differential viable count. They are, therefore, suitable for both general practitioner and hospital work including the screening of ante-natal specimens for symptomless bacteriuria.

For full details, the original papers should be consulted.